Oxidation of adjacent cysteine residues by PHGPx
نویسندگان
چکیده
Matilde Maiorino, Antonella Roveri, Louise Benazzi, Valentina Bosello, Pierluigi Mauri, Stefano Toppo, Silvio C.E. Tosatto, and Fulvio Ursini Department of Biological Chemistry and 3 Department of Biology and CRIBI Biotechnology Centre, Viale G. Colombo, 3, University of Padova, I-35121 Padova, Italy, Institute for Biomedical Technologies, National Research Council, Viale Fratelli Cervi 93, I-2090 Segrate (Milano), Italy
منابع مشابه
Variants of ribonuclease inhibitor that resist oxidation BYUNG - MOON KIM , L .
Human ribonuclease inhibitor ~hRI! is a cytosolic protein that protects cells from the adventitious invasion of pancreatictype ribonucleases. hRI has 32 cysteine residues. The oxidation of these cysteine residues to form disulfide bonds is a rapid, cooperative process that inactivates hRI. The most proximal cysteine residues in native hRI are two pairs that are adjacent in sequence: Cys94 and C...
متن کاملAdjacent cysteine residues as a redox switch.
Oxidation of adjacent cysteine residues into a cystine forms a strained eight-membered ring. This motif was tested as the basis for an enzyme with an artificial redox switch. Adjacent cysteine residues were introduced into two different structural contexts in ribonuclease A (RNase A) by site-directed mutagenesis to produce the A5C/A6C and S15C/S16C variants. Ala5 and Ala6 are located in an alph...
متن کاملOxidation of p53 through DNA Charge Transport Involves a Network of Disulfides within the DNA-Binding Domain
Transcription factor p53 plays a critical role in the cellular response to stress stimuli. We have seen that p53 dissociates selectively from various promoter sites as a result of oxidation at long-range through DNA-mediated charge transport (CT). Here, we examine this chemical oxidation and determine the residues in p53 that are essential for oxidative dissociation, focusing on the network of ...
متن کاملOverexpression of PHGPx inhibits hydroperoxide-induced oxidation, NFkappaB activation and apoptosis and affects oxLDL-mediated proliferation of rabbit aortic smooth muscle cells.
Rabbit abdominal aortic smooth muscle cells (SMC) were stably transfected with the cDNA of porcine phospholipid hydroperoxide glutathione peroxidase (PHGPx) by means of a retroviral gene transfer technique, to create a model for studying cellular processes relevant to atherogenesis. The transfected cells (SMC/PHGPx) had approximately 4-fold higher PHGPx activity when cultured in the presence of...
متن کاملQuantitative mapping of oxidation-sensitive cysteine residues in SERCA in vivo and in vitro by HPLC-electrospray-tandem MS: selective protein oxidation during biological aging.
The selective reversible S-glutathiolation of specific SERCA (sarcoplasmic/endoplasmic-reticulum Ca2+-ATPase) cysteine residues represents a novel physiologic pathway of NO (nitric oxide)-dependent arterial smooth muscle relaxation [Adachi, Weisbrod, Pimentel, Ying, Sharov, Schöneich and Cohen (2004) Nat. Med. 10, 1200-1207]. This mechanism may be impaired through the irreversible oxidation of ...
متن کامل